Characteristics of mRNA level of interleukin 12 and intercellular adhesion molecule 1 and monocyte chemotactic protein 3 in human nasal epithelial cells.
- Author:
Xuewei ZHU
1
;
Dongdong ZHU
;
Kewei SUN
;
Zhen DONG
Author Information
1. Department of Otolaryngology Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Chemokine CCL7;
metabolism;
Epithelial Cells;
metabolism;
Humans;
Intercellular Adhesion Molecule-1;
metabolism;
Interleukin-12 Subunit p35;
metabolism;
Nasal Mucosa;
cytology;
metabolism;
RNA, Messenger;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(23):1068-1070
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the mRNA level of IL-12, ICAM-1 and MCP-3 in human nasal epithelial cells.
METHOD:Firstly, human primary nasal epithelial cells were cultured, and then 4 pairs of primers were designed for detecting mRNA level of IL-12, ICAM-1 and MCP-3. The 938 bp PCR products of GAPDH were used as internal standards. The mRNA expression levels of IL-12, ICAM-1 and MCP-3 in primary nasal epithelial cells was measured with semi-quantitative reverse transcription-polymerase chain reaction.
RESULT:The round or irregular primary nasal epithelial cells were observed sticking to the bottom of cell culture plates under 400 times optical microscope. The expressions of IL-12 p35, ICAM-1 and MCP-3 mRNA were found in primary nasal epithelial cells while IL-12 p40 subunit was not detected.
CONCLUSION:IL-12 p35, ICAM-1 and MCP-3 mRNAs are expressed in primary nasal epithelial cells, whereas effective IL-12 with integrity is not present in nasal epithelial cells.
