Idenfication of microRNAs profiles in nasopharyngeal carcinoma.
- Author:
Guohui NIE
;
Feng LIU
;
Yilin LIN
;
Jing CHEN
;
Zhendong YU
;
Xiaoqing LI
;
Hongfang DUAN
;
Ruijing LU
;
Chunjuan ZHAO
;
Hongyi HU
;
Xueshuang MEI
- Publication Type:Journal Article
- MeSH:
Carcinoma;
Gene Expression Profiling;
Humans;
MicroRNAs;
genetics;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
genetics;
Oligonucleotide Array Sequence Analysis
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(13):975-978
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To filtrate and prove the different microRNAs (miRs) profiles in nasopharyngeal carcinoma.
METHOD:Screening the different expressions of miRs between nasopharyngeal carcinoma and the inflammatory tissues by the application of expression profiling of chip high-throughput and large-scale microarray analysis. Then we used RT-QPCR technology to prove the accuracy of screening results.
RESULT:There were significant expression differences of miRs between nasopharyngeal carcinoma and the control tissues, 144 human miRs had 2 or more fold the difference ratio. Compared with the inflammatory tissues, we have found that miRs-34b, miRs-449b and miRs-7-1 significantly low expressed in nasopharyngeal carcinoma, yet miRs-125b, miRs-184, miRs-196b, miRs-205 and miRs-24-1 expressed high. The results were consistent with the microarray analysis.
CONCLUSION:The difference expressed miRs might be closely related to the process of nasopharyngeal carcinoma, and the research on miRs profiles maybe provide a powerful target basis for early diagnosis and therapy of nasopharyngeal carcinoma.
