The influence of the single different radiation dose and time on the microscopic structure and ultrastructure of Balb/c mice.
- Author:
Chen YANG
;
Wei ZHANG
;
Xiaolong LIU
;
Yong LIANG
;
Peng LI
;
Yi ZHANG
;
Yawei YUAN
- Publication Type:Journal Article
- MeSH:
Animals;
Cochlea;
radiation effects;
ultrastructure;
Dose-Response Relationship, Radiation;
Male;
Mice;
Mice, Inbred BALB C;
Microscopy, Electron, Scanning;
Radiation Dosage;
Time Factors
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(13):979-982
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the influence of the single different radiation dose and time on the microscopic structure and ultrastructure of Balb/c Mice.
METHOD:Sixteen four-week-old mice were randomly divided into four groups, control group and three experimental groups, then every experimental group was randomly divided into two groups which were respectively sacrificed on the third and seventh day after exposure to different doses of 8, 12, 16 Gy radiation, then paraffin embedding, tissue section, HE staining and scanning electron microscope, observation were performed after a cochlear specimen collected.
RESULT:Inner hair cells and outer hair cells arranged in order, no lodging, disorder or missing in control group under scanning electron microscope. Slight lodgy or disorder occured in inner cells and outer hair cells were missing sometimes in exposed animals,which meat irrespective of irradiation dose.
CONCLUSION:Slight changes of ultrastructure of Balb/c Mice occured in the early period when given eaqual to or less than 16 Gy radiation.
