Preliminary mechanism of paclitaxel enhanced radiation sensitivity for nasopharyngeal carcinoma cells.
- Author:
Xi CHEN
;
Zhenwei ZOU
;
Xiaofen PAN
;
Jingjing MOU
;
Gang PENG
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Carcinoma;
Cell Cycle;
drug effects;
Cell Line, Tumor;
Humans;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
pathology;
Paclitaxel;
pharmacology;
Protein Tyrosine Phosphatase, Non-Receptor Type 6;
metabolism;
Radiation Tolerance;
drug effects
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(15):1129-1136
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the radiation-sensitizing function and preliminary mechanism of paclitaxel in radiation-resistant nasopharyngeal carcinoma cells.
METHOD:X-ray dose fractionated irradiation technology to build radiation-resistant subline of nasopharyngeal carcinoma; CNE-2S1 was treated with paclitaxel alone or combined with radiation therapy, while control group treated with radiation therapy; cell colony formation assay was used to observe sensitizing effect of paclitaxel on radiotherapy; flow cytometry analysis was used to analyze cell cycle distribution and apoptosis ratio of different treatment groups; immunoblotting was used to analyze SHP-1 expression levels of different treatment groups.
RESULT:Nasopharyngeal carcinoma cells resistant to radiation was successfully established; cell colony formation assay showed that paclitaxel has obvious sensitizing effect on radiotherapy; FACS results showed that: CNE-2S1 treated by paclitaxel were arrested in G2M phase; paclitaxel and radiotherapy treatments significantly improved the CNE-2S1 apoptosis ratio; Western blot results showed that paclitaxel and combined radiotherapy can reduce the CNE-2S1 cells SHP-1 expression levels.
CONCLUSION:Paclitaxel enhanced radiation therapy for nasopharyngeal carcinoma cells resistant to radiation, and SHP-1 may be involved in this progress.
