Effect of platelet-rich plasma on AnxA1 gene and PPARγ gene of adipose-derived mesenchymal stem cells of rabbit
10.3760/cma.j.issn.1671-0290.2019.01.018
- VernacularTitle:富血小板血浆对兔脂肪间充质干细胞AnxA1基因和PPARγ基因表达的影响
- Author:
Siming WEI
1
;
Gang HU
;
Huizhong GUO
;
Yuquan WANG
;
Le KANG
;
Hongzhi QIN
Author Information
1. 大连医科大学附属第一医院整形科
- Keywords:
Platelet-rich plasma;
Stem cells;
Gene expression;
AnxA1 gene;
PPARγ gene;
Adipose-derived mesenchymal stem cells
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2019;25(1):64-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of platelet-rich plasma(PRP) on starting of AnxA1 gene and PPARγ gene of adipose-derived stem cells (ADSCs) in rabbit.Methods Epididymal adipose tissue stem cells from New Zealand white rabbits,and the cells identified by morphology and inducing differentiation,the cells were cultured to the fourth generation,PRP and PPP (platelet-poor plasma) were prepared by traditional centrifugal method from abdominal aortic of rabbit;ADSCs were cultured in culture medium containing PRP (experimental group),PPP (control group) and all medium (blank group) for each 5％ for 24 h,48h and 72 h.Cells of each group were dissociated and total RNA extracted.AnxA1 gene and PPARγ gene were detected by RT-PCR.Results Primary ADSCs of rabbit grew in the way of long spindle swirly.The results of oil red O and alizarin red staining of the ADSCs were positive.AnxA1 gene and PPARγ gene of experimental group significantly increased from the result of RT-PCR (P＜0.05).Conclusions PRP can promote proliferation of the ADSCs of rabbit and increase the expression of AnxA1 gene and PPARγ gene significantly.
