The clinical value of plasma cell-free DNA
10.3760/cma.j.issn.1009-9158.2019.04.016
- VernacularTitle:血浆游离DNA检测临床意义的研究进展
- Author:
Yulong LI
1
;
Jianxun HE
;
Xiaoli ZENG
;
Hui YUAN
Author Information
1. 首都医科大学附属北京安贞医院检验科北京市心肺血管疾病研究所
- Keywords:
Cell-free nucleic acids;
Molecular diagnostic techniques;
Biomarkers
- From:
Chinese Journal of Laboratory Medicine
2019;42(4):318-322
- CountryChina
- Language:Chinese
-
Abstract:
As a new noninvasive molecular biomarker, plasma cell-free DNA (cfDNA) has potential value in early diagnosis, prognosis evaluation, therapeutic monitoring. Now lots of methods can be used to detect cfDNA including SSCP-PCR, RFLP-PCR, sequencing, and digital PCR. Different methods could be applied in screening of unknown mutations, analysis of genetic polymorphism, and anlysis of specific mutations. cfDNA can be applied in early screening and evaluation in cancers, prenatal test, cardiovascular diseases, and transplantation. This manuscript reviewed the detective value of cfDNA on different diseases.
