Ultra-high performance liquid chromatography tandem mass spectrometry method for detection of α-hydroxybutyrate
10.3760/cma.j.issn.1009-9158.2019.01.012
- VernacularTitle:超高效液相色谱串联质谱检测α-羟基丁酸方法的建立
- Author:
Zhonggan JIN
1
;
Ming ZONG
;
Zhiyan FU
;
Zhihan YE
;
Lieying FAN
Author Information
1. 上海市临床检验中心 200126
- Keywords:
Butyric Acids;
Chromatography,High Pressure Liquid;
Insulin resistance
- From:
Chinese Journal of Laboratory Medicine
2019;42(1):57-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for detecting α-hydroxybutyrate (α-HB) in serum.Methods Electrospray ionization negative ion and multiple reaction monitoring mode were used to detect serum α-HB.The linearity,low limits of quantification,precision,recovery and interference of UHPLC-MS/MS were evaluated.The reference interval of this method was established in 130 serum samples (62 males and 68 females) from Shanghai East Hospital.Dixon method was used to judge the outliers and K-S test was used to analyze the data normality.The standard curve was scored by linear regression analysis.Results The total run time was 4 min of UHPLC-MS/MS method for the determination of α-HB.It has a good linear relationship in the range of 0.5-40.0 mg/L(r=0.999 4);the low limit of quantification was 0.5 mg/L;the in-batch and inter-batch coefficient of variation precision were less than 4.1％ and 6.3％,respectively;the recovery ranged between 95.8％ and 103.8％.Hemolytic samples (about 5 g/L hemoglobin),lipemic samples (about 12 mmol/L triglyceride),icteric samples (about 150 μmol/L total bilirubin) had no significant interference to the detection.The reference range of the apparent healthy population was 1.46-6.48 mg/L.Conclusions A method for the determination of serum α-HB by UHPLC-MS/MS was established.The method was simple,rapid,and could be used for the detection of clinical samples.
