Differential expression of exosomal miRNAs between androgen-dependent prostate cancer cell and androgen-independent prostate cancer cell
10.3760/cma.j.issn.1009-9158.2019.01.007
- VernacularTitle:外泌体miRNAs在前列腺癌细胞激素非依赖转化中的差异表达分析
- Author:
Lin LEI
1
;
Yueyun MA
;
Yun YE
;
Xiaoke HAO
Author Information
1. 空军军医大学附属西京医院检验科
- Keywords:
Prostatic neoplasms;
Exosomes;
microRNAs;
High-throughput nucleotide sequencing
- From:
Chinese Journal of Laboratory Medicine
2019;42(1):26-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective Studying the differential expression of exosomal miRNAs between androgen-dependent prostate cancer cell and androgen-independent prostate cancer cell,in order to further elucidate the mechanism of androgen-independent prostate cancer and find new targets for its treatment.Methods Prostate cancer androgen-dependent LNCaP cell and prostate cancer androgen-independent LNCaP-AI + F cell (induced by androgen and flutamide) were selected as the study subjects.Illumina HiSeqTM 2500 was used to perform high-throughput sequencing between the two groups.The differentially expressed exosomal miRNAs was verified by quantitative real-time PCR(qRT-PCR).The difference was statistically significant by t-test.Results Through the analysis of high-throughput sequencing results,thirteen molecules were screened increased in extracellular exosomes of the androgen-independent cell,including miR-7-5p,let-7a-5p,miR-375,miR-423-3p,miR-378a-3p,and miR-92a-3p,etc.Among them,miR-7-5p was verified by quantitative real-time PCR to be up-regulated by 19.52-fold (t=9.857,P=0.001).Conclusion Differentially expressed exosomal miRNAs may predict the development of androgen-independent prostate cancer and may further regulate the development of androgen-independent prostate cancer.
