Selection of the appropriate pore size, surface modification technique for 3D printed tracheal graft and evaluation of its property
10.3760/cma.j.issn.1001-4497.2019.03.011
- VernacularTitle:3D打印气管的孔径遴选、表面修饰与性能评价
- Author:
Yibo SHAN
1
,
2
,
3
;
Yiwei FAN
;
Jianfeng LI
;
Hongcan SHI
;
Shu PAN
;
Fangbiao ZHANG
Author Information
1. 扬州大学医学院 225001
2. 江阴市人民医院胸心外科 214400
3. 扬州大学转化医学中心 225001
- Keywords:
3D printing;
Trachea;
Polycaprolactone;
Surface modifications;
Evaluation
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2019;35(3):171-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare 3D printed porous tracheal graft fabricated by PCL and to select the appropriate pore size and surface modification techniques,in order to explore its effect on cell behavior.Methods The PCL porous tracheal graft was prepared by 3D printing technology and biomechanical properties of the graft were measured by means of longitudinal tension,radial compression and three-point bending test.The porous grafts were surface-modified through hydrolysis,amination and nanocrystallization treatment and then characterized by energy dispersive spectroscopy(EDS).The effect of different pore sizes and surface modifications on the cell proliferation behavior was evaluated by CCK-8 and scanning electron microscopy (SEM).Results The 3 D printed porous tracheal graft had similar morphology with the native tracheas(P ＞ 0.05) and better biomechanical properties(P ＜0.05).It was more suitable for cell adhesion and proliferation when the pore size is 200 μm (P ＜ 0.05).Compared to hydrolysis and amination,nanocrystallization treatment successfully improved the cytotropism of the 3D printed tracheal graft(P ＜ 0.05).Conclusion 3 D printed porous tracheal graft shows favorable biomechanical properties.The appropriate pore size of the 3D printed porous tracheal graft is 200 μm and the appropriate surface modification techniques is nanocrystallization.
