Investigation effect of induction time on proliferation rate of induced Schwann-like cells from adipose derived stem cells
10.3760/cma.j.issn.1001-2036.2019.02.012
- VernacularTitle:诱导时间对脂肪干细胞来源的类许旺细胞增殖率的影响
- Author:
Zhaowei HUANG
1
;
Zhaowei ZHU
;
Shuqia XU
;
Xunxun LIN
;
Bing HAN
;
Xiangxia LIU
;
Yangbin XU
Author Information
1. 中山大学附属第一医院整形外科
- Keywords:
Adipose stem cells;
Schwann-like cells;
Schwann cells;
Induction;
Cell proliferation
- From:
Chinese Journal of Microsurgery
2019;42(2):150-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective Comparison of induction time on the proliferation of induced adipose-derived stem cells (ADSC) to differentiate into Schwann-like cells (iSC).Methods From March,2017 to October,2018,ADSCs were isolated from inguinal adipose tissue of healthy adult female SD rats.Flow cytometry was performed to detect ADSC positive markers CD29,CD90 and negative marker CD45.iSC induction medium was used to culture ADSC.S-100 and GFAP were detected by immunofluorescence staining to confirm that ADSC had differentiated into iSC.Morphological changes of cells were observed by inverted microscope on day 1st,4th,7th,10th,13rd,16th and 19th after induction.MTS assay was used to evaluate cell proliferation ability.Tunel staining was applied to assess cell apoptosis.Results Both S100 and GFAP were expressed in iSC.On day 7th,the cell proliferation rate was significantly slower than that before induction (A value was 0.330±0.020 vs.0.400±0.004,P＜0.05).It was negatively correlated with induction time.On day 19th,the proliferation rate of iSC was lower than 50％ of the proliferation rate before induction (A value was 0.016±0.003 vs.0.400±0.004,P＜0.05).Apoptosis of iSC was more obvious than ADSC at the same time point.Conclusion The proliferation ability of ADSC-induced iSC is optimal within 7 days after induction.
