Oral immunization of BALB/ c mice with Helicobacter pylori lysate combined with dmLT adjuvant for the induction of mucosal and system immune responses
10.3760/cma.j.issn.0254-5101.2019.04.009
- VernacularTitle:BALB/c小鼠口服幽门螺杆菌裂解物配伍dmLT佐剂诱导黏膜局部及系统性免疫应答
- Author:
Youxiu ZHONG
1
;
Jing CHEN
;
Yu LIU
;
Chongfa TANG
;
Bo WEI
;
Meiying LIU
Author Information
1. 国药中生生物技术研究院有限公司第五研究室
- Keywords:
Sydney strain 1 lysate;
Double mutant heat-labile toxin (dmLT);
BALB/ c mouse;
Immune response;
Helicobacter pylori vaccine
- From:
Chinese Journal of Microbiology and Immunology
2019;39(4):298-304
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effects of oral immunization with Helicobacter pylori (Hp) lysates in combination with mucosal adjuvant dmLT (double mutant heat-labile toxin) against Hp infection in a BALB/ c mouse model and to analyze the features of induced immune responses. Methods BALB/ c mice were orally immunized with Hp lysate (Sydney strain 1, SS1 strain) and dmLT adjuvant, and then innoculated with live Hp strains through oral gavage. A control group was set up by oral administration of normal saline (200 μl/ mouse). The colonization of Hp strains in the stomachs of mice was measured six weeks after bacterial inoculation. Samples of serum, spleen, mesenteric lymph node (MLN), small intes-tine, cecum and feces were collected from mice to analyze the features of induced immune responses. Re-sults The colonization of Hp strains in the stomachs of the immunized mice was significantly decreased as compared with that of the control group. Increased specific IgG antibody responses which were predominantly of IgG1 subtype were detected in the serum samples of the immunized mice and the IgG1 / IgG2a ratio was significantly higher than that of the control group. Elevated secretory IgA (sIgA) was detected in the samples of small intestine, cecum and feces in the immunization group, especially in the small intestine samples, while no significant change in sIgA secretion was observed in the control group. The percentages of IL-17+CD4+ T cells in spleen and mesenteric lymph nodes of the immunization group were significantly higher than those of the control group. Conclusions Oral immunization with Hp lysates in combination with adjuvant dmLT induced mucosal and systemic immune responses and enhanced the resistance to Hp colonization in BALB/ c mice, which was associated with the significantly increased Th17 immune responses and Th2 polari-zation. This study provided reference for further evaluation of dmLT as a mucosal adjuvant in the develop-ment of recombinant protein vaccines against Hp infection.
