Rapid establishment of a mouse model of MERS-CoV pseudovirus infection based on DPP4 transduction
10.3760/cma.j.issn.0254-5101.2019.04.002
- VernacularTitle:基于DPP4转导快速建立MERS-CoV假病毒感染小鼠模型
- Author:
Junwei NIU
1
;
Ying ZHAN
;
Yao DENG
;
Wenjie TAN
Author Information
1. 中国疾病预防控制中心病毒病所 国家卫生健康委员会生物安全重点实验室
- Keywords:
Pseudotyped Middle East respiratory syndrome coronavirus;
Animal model;
Mouse;
Firefly luciferase;
Optical imaging
- From:Chinese Journal of Microbiology and Immunology
2019;39(4):250-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To rapidly establish a mouse model for optical imaging of the dynamical process of pseudotyped Middle East respiratory syndrome coronavirus (MERS-CoV) infection. Methods In vitro expression in HeLa cells and functions of hDPP4 encoded by recombinant adenovirus Ad5 and pseudo-typed MERS-CoV were verified. The recombinant adenovirus expressing hDPP4 (Ad5-hDPP4) was injected into BALB/ c mice, which were then injected with pseudotyped MERS-CoV expressing firefly luciferase at a titer of 3×107 TCID50(50％ tissue culture infective dose) via intrathoracic (I. T. ) or intraperitoneal (I. P. ) injection. MERS-CoV infection and tissue distribution were observed using optical imaging techniques. Re-sults hDPP4 and firefly luciferase were efficiently expressed in HeLa cells. In BALB/ c mice injected with Ad5-hDPP4 via I. P. , firefly luciferase expression were detected in abdomen between 48-96 h after pseudo-typed MERS-CoV infection. The expression of firely luciferase was also detected in chests of BALB/ c mice injected with Ad5-hDPP4 via I. T. around 48 h after pseudotyped MERS-CoV infection. Conclusions This study reported a simple and rapid method for establishing a mouse model for conveniently and dynamically monitoring pseudotyped MERS-CoV infection, which might provide an effective means for in vivo evaluation of neutralizing antibodies or entry inhibitors by visualization with optical imaging techniques.
