Biological characteristics of coxsackievirus A6 clinical isolates
10.3760/cma.j.issn.0254-5101.2019.04.001
- VernacularTitle:柯萨奇病毒A6临床分离株生物学特性分析
- Author:
Xin HE
1
;
Ting YANG
;
Hua LI
;
Lei YUE
;
Xia SONG
;
Tianhong XIE
;
Weijie GAO
;
Zhongping XIE
Author Information
1. 中国医学科学院北京协和医学院医学生物学研究所
- Keywords:
Coxsackievirus A6 (CVA6);
Hand;
foot and mouth disease (HFMD);
Vaccine;
Bi-ological characteristics
- From:
Chinese Journal of Microbiology and Immunology
2019;39(4):241-249
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the biological characteristics of clinical isolates of coxsackievir-us A6 (CVA6), a pathogen of hand,foot and mouth disease (HFMD), and to provide reference for vaccine development. Methods CVA6 strains were isolated from 21 stool and throat swab specimens of patients with HFMD in Yunnan Province and then identified. Their growth characteristics, plaque morphology and virulence to suckling mice were analyzed. Results Five CVA6 strains, named CVA6-129, CVA6-113, CVA6-57, CVA6-94 and CVA6-162, were isolated and all belonged to D3 subtype. Only the CVA6-129 strain could proliferate rapidly in Vero and KMB17 cells and the proliferation peaked 30 h after inoculation. The infectious titer of the CVA6-129 strain was 7. 54 lgCCID50 (50％ cell culture infective dose) / ml in KMB17 cells. Different morphologies of plaques were formed by the CVA6-129 strain in Vero and KMB17 cells at the same time points, which were small and round with clear edges in Vero cells, and large and irregular with blurry edges in KMB17 cells. Suckling mice were susceptible to CVA6 via intramuscular and intraperito-neal injection. The most common symptoms in infected suckling mice were reduced mobility, hind limb pa-ralysis and quadriplegia. CVA6 infection could result in death in severe cases. Conclusions This study isolated five CVA6 strains from a number of clinical samples of suspected HFMD cases, of which the CVA6-129 strain showed potential as a vaccine candidate.
