Cross-reactive immune responses to Mycobacterium vaccae, Mycobacterium tuberculosis and Bacillus Calmette-Guerin
10.3760/cma.j.issn.0254-5101.2019.03.010
- VernacularTitle:母牛分枝杆菌与结核分枝杆菌和卡介苗的交叉免疫应答研究
- Author:
Tongyang XIAO
1
;
Xiaoqin LI
;
Yuhan YAN
;
Na LI
;
Li WAN
;
Haican LIU
;
Machao LI
;
Xiuqin ZHAO
;
Zhiguang LIU
;
Kanglin WAN
Author Information
1. 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室传染病诊治协同创新中心
- Keywords:
Mycobacterium vaccae;
Mycobacterium tuberculosis;
Bacillus Calmette-Guerin;
Cross immunity;
Vaccine
- From:
Chinese Journal of Microbiology and Immunology
2019;39(3):212-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cross-reactive immune responses to Mycobacterium vac-cae (M. vaccae), Mycobacterium tuberculosis (M. tuberculosis, H37Rv) and Mycobacterium bovis Bacillus Calmette-Guerin ( BCG) for providing reference for the development of new vaccines with M. vaccae. Meth-ods M. vaccae (ATCC95051), M. tuberculosis (H37Rv) and BCG (China strain) were cultured on L-J solid media and harvested. Total bacterial protein antigens prepared by ultrasonic disruption were used to im-munize BALB/c mice. IgG antibodies in serum samples were detected with enzyme-linked immunosorbent assay ( ELISA) to evaluate humoral immune responses. Cellular immunity was assessed by detecting various cytokines with cytokine release assay ( CRA) . Results The mice that were respectively immunized with the three mycobacterial antigens could produce high titers of antibodies ( IgG) and high levels of IFN-γand IL-2, but low levels of IL-4 and IL-10. Results of the cross reactivity tests showed that ATCC95051, H37Rv and BCG were able to cross-react with the immunized mice, and all of them induced high levels of IFN-γ, IL-2 and IgG antibodies. Conclusions The three Mycobacteria mainly elicited Th1 immune responses. There were cross-reactive immune responses to M. vaccae, M. tuberculosis and BCG, which might provide ref-erence for using M. vaccae in the development of new anti-tuberculous vaccines.
