Etiological characteristics and molecular typing of Yersinia enterocolitica in Henan province
10.3760/cma.j.issn.0254-5101.2019.03.004
- VernacularTitle:河南省小肠结肠炎耶尔森菌病原学特征和分子分型研究
- Author:
Yujiao MU
1
;
Baifan ZHANG
;
Jingjing PAN
;
Junrong LIANG
;
Xin WANG
;
Shengli XIA
;
Xueyong HUANG
Author Information
1. 河南省疾病预防控制中心传染病预防控制所
- Keywords:
Yersinia enterocolitica;
Serotyping;
Virulence gene;
Pulsed-field gel electrophoresis;
Molecular typing
- From:
Chinese Journal of Microbiology and Immunology
2019;39(3):180-184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution and etiological characteristics of Yersinia en-terocolitica in Henan province between 2011 and 2017 and to analyze the homology among pathogenic strains. Methods A total of 12728 samples, including stool specimens from patients with diarrhea and domestic animals, flies, and smear specimens from raw and cooked meat products, were collected. Cold enrichment method was used to isolate Yersinia enterocolitica. The isolated strains were analyzed by biochemical identifi-cation, biotyping, serotyping and virulence gene detection with PCR. Pulsed-field gel electrophoresis ( PFGE) was performed for molecular typing of pathogenic strains. Results There were 390 strains of Yersinia enterocolitica isolated from the 12728 specimens with a detection rate of 3. 06％, including 13 hu-man strains and 377 animal strains. Most of the strains were isolated from pig and chicken feces and both ac-counted for 25. 13％ (98/390). The predominant biotype was 1A and the serotypes of the strains were main-ly O : 5 and O : 8. Results of the virulence gene analysis showed that 21 strains of O : 3 serotype were path-ogenic, including one human strain and 20 pig strains. After NotⅠdigestion, these pathogenic strains were divided into three band types with a band similarity of 94％-100％. Conclusions Yersinia enterocolitica ex-isted in both human population and many kinds of animals in Henan province. Pig was the main host of path-ogenic strains and there was a high homology among these strains.
