IL-10 promotes Chlamydia muridarum infection in mice through inhibiting IFN-γand IL-2 expression
10.3760/cma.j.issn.0254-5101.2019.02.009
- VernacularTitle:IL-10通过抑制IFN-γ和IL-2表达促进鼠衣原体感染
- Author:
Qingzhi HE
1
;
Mengting HE
;
Xin WANG
;
Xiaoqing LUO
;
Yimou WU
;
Guangming ZHONG
Author Information
1. 南华大学药学与生物科学学院
- Keywords:
Chlamydia trachomatis;
IL-10;
Cytokine;
Immunity
- From:
Chinese Journal of Microbiology and Immunology
2019;39(2):126-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the possible molecular mechanism of IL-10 in promoting Chlamydia muridarum infection in mice. Methods C57BL/6 wild-type and IL-10 gene knockout ( IL-10-/-) mice were infected with Chlamydia muridarum. Indirect immunofluorescence assay was used to detect the growth of Chlamydia muridarum in the intestinal and genital tracts. The severity of genital diseases was assessed by hydrosalpinx scoring. Expression of IFN-γand IL-2 in blood was measured by ELISA. Re-sults Compared with the wild-type group, Chlamydia clearance in the intestinal and genital tracts of IL-10-/- mice was significantly faster, and the expression of IFN-γ and IL-2 increased significantly. In addi-tion, wild-type mice showed more serious hydrosalpinx. Conclusions IL-10 delays Chlamydia trachomatis clearance and promotes Chlamydia infection through inhibiting the expression of IFN-γ and IL-2, which ag-gravates hydrosalpinx.
