Identification and biological characteristics of an Actinomyces europaeus strain
10.3760/cma.j.issn.0254-5101.2019.02.007
- VernacularTitle:一株临床分离的欧洲放线菌的鉴定及生物学特性研究
- Author:
Mingyan SUN
1
;
Qianqian WU
;
Baoqiang WANG
;
Nan WANG
;
Yanxia LIU
;
Yuanyong TAO
Author Information
1. 潍坊医学院附属医院检验科 261031
- Keywords:
Actinomyces europaeus;
Actinomyces;
Actinomycosis;
MALDI-TOF MS;
16S rRNA
- From:Chinese Journal of Microbiology and Immunology
2019;39(2):120-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the methods for identifying Actinomyces europaeus and to analyze its biological characteristics in order to provide a basis for the diagnosis of actinomycosis. Methods Pus speci-mens collected from patients were used for bacterial culture and then analyzed with Gram staining. VITEK 2 Compact automatic microbiological analyzer was used for species identification. Drug susceptibility test was per-formed with E-test. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry ( MALDI-TOF MS) was used to identify the isolated strain. The common primers of 16S rRNA were used for amplification fol-lowing DNA extraction, and the product of PCR was sequenced after recovery and purification. Homology analy-sis was conducted using the sequence in GenBank database. Results The drug susceptibility test showed that the strain was sensitive to penicillin, piperazolin/taclobatan, and ceftriaxone, but resistant to ciprofloxacin. MALDI-TOF MS and 16S rRNA gene assay identified the strain as Actinomyces europaeus. Conclusions MALDI-TOFMS and 16S rRNA could be used to identify Actinomyces europaeus and are of great significance for the diagnosis of actinomycosis.
