Obtaining vacuolar protein sorting 34 gene in Sporothrix globosa by RACE method and analysis of its expression during the mycelium-to-yeast transition
10.3760/cma.j.issn.0254-5101.2019.01.008
- VernacularTitle:球形孢子丝菌vps34基因序列的获得及其在双相型表达差异的初探
- Author:
Yan CUI
1
;
Xinyao QU
;
Yang SONG
;
Shanshan LI
Author Information
1. 吉林大学第一医院皮肤性病科
- Keywords:
Sporothrix globosa;
Sporotrichosis;
Vacuolar protein sorting 34;
Rapid amplification of cDNA ends;
Quantitative reverse transcription PCR;
Dimorphic transformation
- From:
Chinese Journal of Microbiology and Immunology
2019;39(1):42-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain the full-length sequence of the vacuolar protein sorting 34 coding gene (vps34) of Sporothrix globosa (S. globosa) and to investigate the role of vps34 gene during the phase transition from mycelium to yeast in S. globosa. -ethods The 3′ end and 5′ end of the vps34 gene of S. globosa were amplified by rapid amplification of cDNA ends ( RACE ) . The obtained sequences were spliced and analyzed by bioinformatics software. Quantitative reverse transcription PCR ( qRT-PCR ) was used to analyze the expression of vps34 gene in mycelial and yeast phases. Results The vps34 gene of S. globosa was 3228 bp in length. The coding sequence was 3000 bp and encoded 999 amino acids with a mo-lecular mass of 111. 49×103 and an isoelectric point of 6. 38. It contained three domains including C2 PI3K class Ⅲ, PI3Ka Ⅲ and PI3Kc Ⅲ. The results of qRT-PCR showed that the expression of vps34 gene in yeast-phase S. globosa was higher than that in mycelial phase at 24 h (P<0. 05), and the greatest difference between them was observed at 48 h (P<0. 01). Conclusions Vps34p participates in the process of dimor-phic transformation of S. globosa. The obtainment of the full-length vps34 gene of S. globosa lays the founda-tions for further study on the function of Vps34p.
