Effects of 2-h Ex Vivo Lung Perfusion on Pulmonary Arterial Endothelium

VernacularTitle:猪肺体外灌注2小时对肺动脉收缩功能的影响
Author: Jingfeng HU ¹ ; Dengyan ZHU ; Yang YANG
Author Information

1. 450052,郑州大学第一附属医院胸外科
Keywords: Lung transplantation; Pulmonary artery; Endothelial cell
From: Chinese Journal of Organ Transplantation 2018;39(12):715-719
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of 2-h Ex Vivo Lung Perfusion (EVLP) on the pulmonary arterial endothelium by comparing the endothelium-dependent relaxation between EVLP group and fresh control group.Methods The lungs from 6 Swedish domestic pigs were divided into two groups:the right lungs as fresh control group and the left lungs as EVLP group.Lungs in EVLP group were perfused for 2 h.Pulmonary artery segments were obtained.The endothelium-dependent relaxation and pulmonary artery smooth muscle contraction were studied in organ bath.Results The contractions induced by U-46619 were 22.4 ± 2.2 mN and 24.5 ± 2.9 mN in two groups.The maximum endothelium dependent relaxations were 97 ± 0.67％ and 97 ± 0.68％ in two groups.The negative logarithm of 50％ relaxation (pEC50) was 6.74 ± 0.07 and 6.76 ± 0.07 in two groups.There was no statistically significant difference between the two groups.Conclusion The function of endothelium and smooth muscle of pulmonary artery was fully preserved after 2-h EVLP.