Effects of NBS1 in MRN complex on gallbladder carcinoma cells
10.3760/cma.j.issn.1007-631X.2019.04.018
- VernacularTitle:MRN复合物中的NBS1对胆囊癌细胞增殖与凋亡的影响
- Author:
Guangyu CHEN
1
;
Yingbin HUANG
;
Yang WANG
;
Rui HE
;
Jun DU
;
Xingyuan JIAO
Author Information
1. 中山大学附属第一医院器官移植中心
- Keywords:
Gallbladder neoplasms;
Cell proliferation;
Apoptosis
- From:
Chinese Journal of General Surgery
2019;34(4):353-357
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of MREll-RAD50-NBS1 complex in normal gallbladder tissues,simple cholecystitis,calculous cholecystitis and gallbladder carcinoma.Methods The expression of MRN complex in different gallbladder lesion tissues were detected by immunohistochemistry.Western blot,Methyl thiazolyl tetrazolium(MTT) assay and flow cytometry were used to detect the influence of apoptosis and proliferation induced by NBS1.Results The differences between the expression of MRE11,RAD50 and different gallbladder lesion tissues were not statistically significant (respectively x2 =2.724,1.697,all P ＞ 0.05).The expression of NBS1 in GBC tissues [15.3％ (9/59)] was prominently lower than that in the normal gallbladder tissues [84.7％ (50/59)],simple cholecystitis [87.8％ (36/41)],calculous cholecystitis [61.2％ (30/49)] (x2 =87.388,P ＜ 0.01).The Western blot results reveal that NBS1 can mediate apoptosis by up-regulate Bax and down-regulate Bcl-2.The MTT assay results showed that the relative absorbance of treatment and control group after 24,48,72 h were[(1.120 ± 0.006)vs.(1.350±0.009),(1.600±0.004)vs.(1.99±0.01),(1.83±0.01)vs.(2.260±0.003)(F=7.659,P ＜0.01).The apoptosis rate in treatment group(17.23％ ± 0.56％)was higher than that in the control group (4.13％ ± 0.67％) (t =9.133,P ＜ 0.01).Conclusion NBS1 is closely related to gallbladder carcinoma.NBS1 can inhibit the proliferation and promote apoptosis of GBC-SD cells.
