Heat shock protein 70 in nuclear translocation involved in DNA repair during ischemia-reperfusion injury
10.3760/cma.j.issn.1007-631X.2019.02.016
- VernacularTitle:热休克蛋白70入核转位参与缺血再灌注损伤的脱氧核糖核酸修复的实验研究
- Author:
Shao ZHANG
1
;
Jing ZHANG
;
Xiaoning ZHANG
;
Chunmei JING
;
Xiang ZHAN
;
Hao LI
Author Information
1. 南华大学第一附属医院胃肠外科
- Keywords:
HSP70Heat-shock proteins;
Reperfusion injury;
DNA repair
- From:
Chinese Journal of General Surgery
2019;34(2):154-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of HSP70 on cell cycle regulation in hepatic IR (ischemia-reperfusion) injury.Methods SD rats were randomly divided into HSP70 inhibitor group (H-/P+),heat shock group (H+/P+),PARP-1 inhibitory group (H +/P-),IR group (PC) and negative control group (NC),respectively.After the IR model was induced,the liver specimen underwent IHC staining to observe the changes of the PARP-1 expression;Co-immunoprecipitation was used to detect the binding of HSPT0 with PARP-1.Results H +/P-was significantly different from H +/P +,H-/P +,PC,NC (P ＜ 0.01);Immunoprecipitation suggested that HSP70 entered into the nucleus to bind PARP-1,and immunofluorescence imaging analysis demonstrated both HSP70 and CyclinD1 expressed at the same timeline.Conclusion Under reversible hepatic ischemia-reperfusion injury,HSP70 enters the nucleus and binds to PARP-1,negatively regulates G2/M phase,blocks cells for DNA replication and recombination,blocks its entry into mitosis,repairs damaged DNA chain;Liver ischemia-reperfusion positively regulates the G1/S phase,promoting hepatocyte regeneration and liver function compensation.
