Diagnostic value of pyruvate kinase M2 gene for papillary thyroid carcinoma in fine-needle aspiration specimens
10.3760/cma.j.issn.1000-6699.2019.04.002
- VernacularTitle:细针穿刺标本M2型丙酮酸激酶基因检测诊断甲状腺乳头状癌的价值
- Author:
Xingyue CAO
1
;
Ya WU
;
Ting XU
;
Hongyan DENG
;
Jing HANG
;
Jianxiang WANG
;
Xiao LI
;
Qing YAO
;
Rong RONG
;
Meiping SHEN
;
Haixia GUAN
;
Xiaohong WU
Author Information
1. 南京医科大学第一附属医院内分泌科 210000
- Keywords:
Papillary thyroid carcinoma;
Pyruvate kinase M2;
Fine-needle aspiration
- From:
Chinese Journal of Endocrinology and Metabolism
2019;35(4):276-281
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diagnostic value of pyruvate kinase M2 ( PKM2) gene expression in papillary thyroid carcinoma ( PTC). Methods Quantitative real-time PCR ( RT-qPCR) was used to detect the expression of PKM2 mRNA in benign thyroid nodules, PTC, and normal thyroid cells around nodules of fine-needle aspiration (FNA) specimens. Immunohistochemistry ( IHC) was used to detect the expression of PKM2 protein in thyroid tissue after thyroidectomy. The receiver operating characteristic curve was constructed to evaluate the diagnostic value of PKM2 in PTC. Results The expression of PKM2 mRNA was detectable in FNA specimens of thyroid nodules,higher in PTC than those in normal thyroid tissue and benign thyroid nodules (P<0.01). PKM2 expression level was correlated with diameter of PTC ( P<0.05) , but had no correlation with lymph node metastasis, BRAFV600E mutation, and American Joint Committe on Cancer( AJCC) stage ( P>0.05) . The expression level of PKM2 mRNA in FNA specimens of thyroid nodules was paralleled with the expression level of PKM2 protein in postoperation pathological tissues. The accuracy, sensitivity, specificity of PKM2 gene in the diagnosis of PTC were 62.8％, 46.9％, and 95.7％, respectively. The accuracy and sensitivity of PKM2 combined with BRAFV600E were increased to 87.6％and 83.7％. Conclusion Detection of PKM2 gene in FNA specimens is highly specific in the diagnosis of PTC, making it a valuable molecular marker for preoperative diagnosis. The combination of PKM2 and BRAFV600E detection shows a higher diagnosis efficiency.
