Association between lncRNA IFNG-AS1 SNP and Hashimoto's thyroiditis
10.3760/cma.j.issn.1000-6699.2019.02.003
- VernacularTitle:长链非编码RNA IFNG-AS1 SNP与桥本甲状腺炎的易感性关联分析
- Author:
Ruhai YI
1
;
Yueqing SU
;
Saifan ZENG
;
Shuhao ZHAO
;
Liyong YANG
Author Information
1. 福州
- Keywords:
IFNG gene;
IFNG-AS1;
Long non-coding RNA;
Single nucleotide polymorphisms;
Hashimoto thyroiditis
- From:
Chinese Journal of Endocrinology and Metabolism
2019;35(2):105-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the association between three single nucleotide polymorphisms( SNPs) ( rs10878724、 rs7980829 and rs11177020 ) of lnc IFNG-AS1 and Hashimoto's thyroiditis ( HT) susceptibility. Methods TaqMan probe technology was used to genotype the selected SNPs in a total of 179 subjects, including 70 HT cases, and 109 controls. The expression levels of lnc IFNG-AS1 and IFNG were detected by SYBR-Green qRT-PCR. Results Compared with control, not only the A allele and AA genotype frequencies of rs10878724 were significantly different in group HT ( P=0. 01, P=0. 003), but also the T allele and TT genotype frequencies of rs7980829 were significantly high in group HT. Haplotype analysis showed that the G-G-A decreased the risk of HT (P=0.04), while the haplotype of A-T-T incresed the risk of HT( P=0.01). The relative expression of both IFNG mRNA and lnc IFNG-AS1 were higher in group HT than in control( P=0. 001,P=0. 013). In HT patients, IFNG mRNA relative expression in both rs7980829-TT and rs1087872-TT were significantly higher than those of other genotypes(P=0.017,P=0.009). Conclusion The SNPs of Inc IFNG-AS1 were correlated with the expression levels of IFNG and lncRNA IFNG-AS1. Noncoding genes should be further screened as potential biomarkers in prediction of HT susceptibility.
