Analysis of extreme obesity in two pedigrees due to leptin receptor mutation
10.3760/cma.j.issn.1000-6699.2019.01.006
- VernacularTitle:两例瘦素受体突变型极端肥胖家系研究
- Author:
Jingya YE
1
;
Zhenzhen FU
;
Wei GUAN
;
Yizhe MA
;
Yingyun GONG
;
Shuai MA
;
Xuan YE
;
Chenxi ZHAO
;
Xiaomei GENG
;
Zhong LI
;
Hui LIANG
;
Hongwen ZHOU
Author Information
1. 南京医科大学第一附属医院内分泌科 210029
- Keywords:
Leptin receptor;
Monogenic obesity;
Compound heterozygous mutation;
Metabolic surgery
- From:
Chinese Journal of Endocrinology and Metabolism
2019;35(1):32-36
- CountryChina
- Language:Chinese
-
Abstract:
This study reported two women with extreme obesity who underwent metabolic surgery due to their mutations in leptin receptor (LEPR).Genomic DNA was extracted from the anticoagulant blood samples of the two patients and their parents.A panel of genes related to metabolic diseases or whole exon sequencing was screened and the results were confirmed by Sanger sequencing.This is the first time that these three mutations in LEPR were reported.Two patients complained insatiety and early-onset obesity since childhood at clinics.Patient 1 was a 39-year-old woman with height 150 cm,weight 130 kg,and BMI 57.8 kg/m2.Serum leptin level was 156.4 μg/L.A homozygous mutation of c.2317G＞T was found in exon 15 of LEPR gene in patient 1,which was descended from her father and mother respectively.Patient 2 was a 37-year-old woman with height 158 cm,weight 167 kg,and BMI 67 kg/m2.Serum leptin level was 193.4 μg/L.Genetic analysis showed compound heterozygous mutations of c.1482delT and c.1892C ＞ A.Her father showed heterozygous c.1482delT mutation,and her mother carried heterozygous c.1892C ＞ A mutation.Two patients all underwent metabolic surgery with body weight reduction of about 22 kg and 40 kg respectively after first six months.However,the follow-up studies showed that the body weight of patient 1 rebounded to pre-surgery level in two years and patient 2 did not further lose weight in the following six months.
