Relationship between mechanism underlying electro-acupuncture-induced improvement of postoperative cognitive dysfunction and mitochondrion-dependent apoptosis in hippocampal neurons of aged rats
10.3760/cma.j.issn.0254-1416.2019.01.010
- VernacularTitle:电针改善老龄大鼠术后认知功能障碍的机制与海马神经元线粒体途径凋亡的关系
- Author:
Xuguang CHEN
1
;
Jinhua HE
;
Yanan LI
;
Qi ZHANG
;
Liang WANG
;
Qiujun WANG
Author Information
1. 河北医科大学第三医院麻醉科
- Keywords:
Electro-acupuncture;
Cognition disorder;
Neurons;
Mitochondria;
Apoptosis
- From:
Chinese Journal of Anesthesiology
2019;39(1):40-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the the relationship between the mechanism underlying electroacupuncture (EA)-induced improvement of postoperative cognitive dysfunction and mitochondrion-depend-ent apoptosis in hippocampal neurons of aged rats.Methods Seventy-two healthy male Sprague-Dawley rats,aged 18 months,weighing 500-550 g,were divided into 3 groups (n =24 each) using a random number table method:control group (group C),operation group (group O) and group EA.EA was performed at Baihui and Dazhui acupoints for 30 min using disperse-dense waves,with a frequency 2/15 Hz and intensity 1 mA,once a day for 5 consecutive days in group EA.Laparotomy was performed under 3％sevoflurane anesthesia after the end of EA stimulation in O and EA groups.Morris water maze test was performed on 1 day before operation and 3 and 7 days after operation to assess the cognitive function.Rats were sacrificed after the end of Morris water maze test,brains were removed,and hippocampal tissues were obtained for detection of apoptosis rate of hippocampal neurons and expression of hippocampal caspase-3 and cytochrome c (Cyt c) by Western blot.Results Compared with group C,the escape latency was significantly prolonged after operation,the frequency of crossing the original platform was reduced,the apoptotic rate of hippocampal neurons was increased,and the expression of caspase-3 and Cyt c was up-regulated in O and A groups (P＜0.05).Compared with group O,the escape latency was significantly shortened after operation,the frequency of crossing the original platform was increased,the apoptotic rate of hippocampal neurons was decreased,and the expression of caspase-3 and Cyt c was down-regulated in EA group (P＜0.05).Conclusion The mechanism by which EA improves postoperative cognitive dysfunction may be related to inhibiting mitochondrion-dependent apoptosis in hippocampal neurons of aged rats.
