Effect of paeonol on expression of HMGB1,IL-1βand apoptosis of cardiomyocytes in rats after myocardial I/R injury
10.3969/j.issn.1009-0126.2019.05.019
- VernacularTitle:丹皮酚对大鼠心肌缺血再灌注损伤中高迁移率族蛋白B1和白细胞介素1β及心肌凋亡的影响
- Author:
Xin HUANG
1
;
Huasheng DING
Author Information
1. 武汉市第四医院手术室
- Keywords:
myocardial ischemia;
reperfusion injury;
high mobility group proteins;
PAEONOL
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2019;21(5):522-524
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of paeonol onexpression of HMGB1and IL-1βand apoptosis of cardiomyocytes in rats after myocardial I/R injury.Methods Fifty-two adult SPF male SD rats were randomly divided into sham operation group,low-dose paeonol group,high dose paeonol group,and I/R injury group(13in each group).The rats in low-dose paeonol group and high dose paeonol group received intraperitoneal paeonol injection before operation(15.30mg/kg,once a day).Serum samples were taken at 2hafter reperfusion.Serum IL-1βlevel was measured by ELISA,myocardial infarction size was measured with TTC staining,expressions of HMGB1,Bcl-2and Bax proteins in myocardial tissue were detected with immunohistochemical staining.Results The myocardial infarction size was significantly smaller in low-dose paeonol group and high dose paeonol group than in I/R injury group(31.04％±2.93％,27.97％±2.84 vs37.23％±3.62％,P＜0.01).The expression levels of IL-1βand HMGB1were significantly higher in I/R injury group,low-dose paeonol group and high-dose paeonol group than in sham operation group and were significantly lower in low-dose paeonol group and high-dose paeonol group than in I/R injury group(P＜0.01).The expression level of IL-1βwas significantly higher while that of Bax protein was significantly lower in low-dose paeonol group and high-dose paeonol group than in in I/R injury group(P＜0.05).Conclusion Paeonol preconditioning can reduce apoptosis of cardiomyocytes,and can thus prevent myocardial I/R injury.
