Ovarian cancer cells targeting fluorescent-magnetic imaging dual-modal nanoprobe:from synthesis to imaging in vitro
10.3760/cma.j.issn.2095-2848.2019.04.007
- VernacularTitle:靶向卵巢癌细胞荧光-磁共振双模态纳米探针的制备及细胞成像
- Author:
Huiting XU
1
;
Jianming NI
;
Cuiping HAN
;
Jingjing LI
;
Wei LI
;
Zhuiyang ZHANG
;
Kai XU
Author Information
1. 南京医科大学附属无锡第二医院核医学科 214002
- Keywords:
Ovarian neoplasms;
Tumor cells,cultured;
Nanoparticles;
Manganese;
Quantum dots;
Antibodies,monoclonal;
Optical imaging;
Magnetic resonance imaging
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2019;39(4):222-226
- CountryChina
- Language:Chinese
-
Abstract:
Objective To fabricate manganese-doped carbon quantum dots(Mn-CDs)@anti-human epididymis protein 4(HE4)monoclonal antibody(Mn-CDs@Anti-HE4 mAb)dual-modal fluorescent-magnetic nanoprobe for ovarian cancer cells targeting imaging,and evaluate its potential on fluorescent imaging and MRL Methods Mn-CDs were synthesized at 150 ℃ with solvothermal method.The average diameter,fluorescent capability and MRI efficiency were determined.The cytotoxicity of Mn-CDs in vitro was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS)assay with HO-8910 ovarian cancer stem cells and EA.hy926 human umbilical vein endothelial cells.Mn-CDs@Anti-HE4 mAb was fabricated with condensation reaction and characterized by ultraviolet(UV)absorption spectra.Fluorescence imaging and MRI in vitro was performed for cancer cell-targeting study.One-way analysis of variance and the least significant difference t test were used to analyze the data.Results The Mn-CDs with diameter of(4.64±0.85)nm showed a well-defined spherical morphology.The fluorescent spectra of Mn-CDs exhibited a typical excitation-dependent behavior with an excitation maximum at 360 nm and emission maximum at 440 nm.The T1 relaxation rate was(3.26±0.04)mmol ? L-1 ? s-1.The cytotoxicity tests in vitro showed that the survival rates of HO-8910 cells and EA.hy926 cells were both significantly different after treated with different concentrations of Mn-CDs(F= 1 947.509,260.174,both P<0.05),and there was no cytotoxicity in both HO-8910 cells and EA.hy926 cells at concentrations of MnCDs within 0-2.5 mg/ml(all P>0.05),while the survival rates of the two kinds of cells were descended with the increasing of concentration within 3.0-4.5 mg/ml(P<0.05).Mn-CDs@Anti-HE4 mAb could target HO-8910 cells on fluorescence imaging and MRI.Conclusions Mn-CDs@Anti-HE4 mAb,with good potential on fluorescence imaging,MRI and targeting ability,is successfully synthesized.It may provide a new method for early diagnosis of ovarian cancer.
