18F-Alfatide n automated preparation based on 18 F-minireactor and microPET/CT imaging in tumor
10.3760/cma.j.issn.2095-2848.2019.04.002
- VernacularTitle:基于新型18F微反应器的18F-阿法肽的自动化制备及microPET/CT显像
- Author:
Jian ZHANG
1
,
2
;
Xiaobao ZHOU
;
Qin SHI
;
Yuyun SUN
;
Yingjian ZHANG
;
Yongping ZHANG
;
Mingwei WANG
Author Information
1. 复旦大学附属肿瘤医院核医学科、复旦大学上海医学院肿瘤学系、复旦大学生物医学 影像研究中心、上海分子影像探针工程技术研究中心 200032
2. 上海师范大学生命与 环境科学学院、教育部资源化学重点实验室、上海市稀土功能材料重点实验室、上海市 高校分子影像探针与传感器重点实验室 200234
- Keywords:
Peptides,cyclic;
Arg-Gly-Asp;
Fluorine radioisotopes;
Chemistry techniques,synthetic;
Breast neoplasms;
Nasopharyngeal neoplasms;
Neoplasm transplantation;
Mice,nude
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2019;39(4):196-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop the automated preparation of 18F-Alfatide II using newly-designed 18F-minireactor and perform 18F-Alfatide D microPET/CT imaging in tumor.Methods The automated preparation of 18F-Alfatide H was developed by using 18F-microreactor and water phase Al18F-chelating method,and the radiochemical yield and quality analysis were measured.The nude mice bearing breast tumor ZR-75-1 and nasopharyngeal tumor CNE1 were established(n = 3 respectively).MicroPET/CT imaging was performed at 0.5,1.0 and 2.0 h after the injection of 18F-Alfatide II.The region of interest(ROI)was depicted and the tumor/muscle(T/M)ratio was calculated.Results 18F-Alfatide II was automatically prepared with the total synthesis time of 40 min,the radiochemical yield of(28±6)%(no decay corrected,n=11),and the radiochemical purity >97%.All quality analysis indexes accorded with the radiopharmaceutical requirements.18F-Alfatide II microPET/CT images of ZR-75-1 and CNE1 tumors were clear due to the high radioactivity uptake of tumor lesions(T/M ratio was greater than 4.0 at 1.0 h after injection).Conclusion Based on the 18F-minireactor,the,8F-Alfatide II can be prepared successfully with short synthesis time and high radiochemical yield,which can help the application studies in 18F-Alfatide II microPET/CT imaging.
