Effect of high voltage electric field on cell growth, apoptosis of human pancreatic cancer cell line PANC-1
10.3760/cma.j.issn.1007-8118.2019.04.011
- VernacularTitle:高压电场对人胰腺癌细胞株PANC-1生长及调亡的影响
- Author:
Mengyun KE
1
;
Fenggang REN
;
Qingshan LI
;
Rongqian WU
;
Yi LYU
Author Information
1. 西安交通大学第一附属医院精准外科与再生医学国家地方联合工程研究中心 陕西省再生医学与外科工程研究中心
- Keywords:
High voltage electric field;
Pancreatic cancer cell;
Growth;
Apoptosis;
Cell membrane;
Organelle
- From:
Chinese Journal of Hepatobiliary Surgery
2019;25(4):288-292
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of high intensity electric field on cell growth,apoptosis and microstructure of human pancreatic cancer cell line PANC-1.Methods The PANC-1 cells in the logarithmic growth period were selected,and cells in the high voltage electrical treatment group were treated with high voltage electric field 250,500,750,1000 V/cm,respectively.The effects of different high voltage electric fields on cell growth and microstructure of PANC-1 cells were determined by cell viability,cell death staining,apoptosis detection,transmission electron microscopy and scanning electron microscopy.Results Compared with control group,the high voltage electric pulse significantly inhibited the growth of PANC-1 cells in the field dependent manner.Moreover,when the field was more than 500 V/cm,the cell viability was significantly decreased (P＜0.05).High voltage electric pulse could induce cell apoptosis.When the field was higher than 750 V/cm,serious necrosis was noticed.In the 1000 V/cm group,the integrity of cell membrane and the structure of organelles was seriously damaged.Conclusion High voltage electric pulse could significantly inhibit the growth of PANC-1 cells and would be a promising method in cancer treatment.
