Effects of transplanting BDNF overexpressed neural stem cells on neurotrophic factor induction hippocampus after radiation exposure
10.3760/cma.j.issn.0254-5098.2019.04.001
- VernacularTitle:过表达脑源性神经营养因子的神经干细胞移植对受照海马内神经营养因子的影响
- Author:
Xue ZHONG
1
;
Qixian ZHANG
;
Jie ZHANG
;
Liesong CHEN
;
Ye TIAN
Author Information
1. 苏州大学附属第二医院放射治疗科苏州大学放射肿瘤治疗学研究所苏州市肿瘤放射治疗学重点实验室 215004
- Keywords:
Radiation-induced cognitive dysfunction;
Neurotrophic factors;
Microglia;
Neural stem cells
- From:
Chinese Journal of Radiological Medicine and Protection
2019;39(4):241-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of neural stem cells (NSCs) overexpressing brainderived neurotrophic factor (BDNF) on the levels of neurotrophic factors and microglia activation in hippocampus after brain irradiation.Methods Hippocampal NSCs were isolated from fetal rat brain and infected with GFP-lentivirus and GFP-BDNF-lentivirus.SD rats were randomized into four groups:control group,irradiated group (R group),GFP-modified NSCs transplantation group with irradiation (R+NSCs group),and GFP-BDNF modified NSCs transplantation group with irradiation (R+BDNF-NSCs group).NSCs were transplanted into the bilateral hippocampus of rats one month after whole brain irradiation at a single dose of 20 Gy.The expressions of BDNF,glial-derived neurotrophic factor (GDNF) and nerve growth factor (NGF) in hippocampus were detected at 2 and 8 weeks after transplantation.The activation of microglia was observed by immunofluorescence.Results At 2 and 8 weeks after transplantation,the expressions of BDNF and NGF proteins in hippocampus of R+BDNF-NSCs group were significantly higher than those of R group (P＜0.05).The activated microglia in the R+NSCs group and the R+BDNF-NSCs group had no decrease compared with R group (P＞ 0.05).Conclusions The transplantation of NSCs overexpressing BDNF promotes the production of BDNF and NGF,which improves the level of neurotrophic factors in hippocampus after radiation.
