The therapeutic effect of G-CSF-mobilized autologous stem cells on radiation pulmonary injury in mice
10.3760/cma.j.issn.0254-5098.2019.03.004
- VernacularTitle:粒细胞集落刺激因子动员自体干细胞对放射性肺损伤的防治作用
- Author:
Huiqin SUN
1
;
Tao WANG
;
Xueli PANG
;
Yu WANG
;
Fengchao WANG
;
Jun HE
;
Qing ZHOU
;
Shuang LONG
;
Xinze RAN
;
Yongping SU
Author Information
1. 陆军军医大学军事预防医学系全军复合伤研究所创伤、烧伤与复合伤国家重点实验室
- Keywords:
Radiation-induced lung injury;
G-CSF;
Autologous stem cell mobilization;
Therapeutic effect;
Mice
- From:
Chinese Journal of Radiological Medicine and Protection
2019;39(3):178-184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of G-CSF-mobilized autologous stem cells in the prevention of radiation pulmonary injury.Methods Mice were divided into control group,irradiation group and treatment group.Mouse model of pulmonary fibrosis was established by exposing chest to a single dose of 14 Gy.Animals in the treatment group received recombinant human G-CSF (250 μg/kg daily for 5 d) before the irradiation in order to mobilize autologous stem cells in vivo.The general condition and mortality were documented after radiation injury.The pathological study with histological scoring,Masson staining and Sirius red staining with polarized light analysis were used to identify lung injury and the potential benefit of stem cell mobilization.Results Local chest irradiation of a single dose of 14 Gy was a suitable dose to create radiation-induced pulmonary fibrosis in mice.The death rate was 37.5％,which mainly happened around 11 weeks after injury.In contrast,all of the animals in G-CSF treated group survived.The ratio of lung to body mass was significantly increased in both irradiation group and treatment group (F =23.20,P＜0.05) around 3 months after the injury,with a higher ratio in irradiation group than that in treatment group (P＜0.05).Histological scoring for alveolar inflammation at 3 months after injury revealed statistically significant difference in irradiation group and treatment group compared with control group (F=11.93,P＜ 0.05).At this time point,the pathological observation showed lung tissue degeneration and necrosis with alveolitis and interstitial inflammation,as well as fibroblasts proliferation and focal collagen deposition in alveolar septa.At 4 month after the injury,the inflammation ininterstitial tissue was receded,but fibrosis and collagen deposition were significantly increased.In addition,at 3 and 4 months afterinjury,the pulmonary fibrosis was aggravated in irradiation group (F=28.73,16.85,P＜0.05),and significantly alleviated in the treatment group (P＜0.05).The similar results were confirmed in collagen content analysis (IOD) by Sirius red staining and image analysis (F =17.70,17.79,P＜ 0.05).Conclusions Autologous mobilization of stem cells could prevent the death of radiation-injured animals possibly by alleviating early lung injury and interstitial inflammation as well as the late pulmonary fibrosis,suggesting a therapeutic potential of autologous stem cell mobilization in radiation pulmonary fibrosis.
