Frequency changes of peripheral blood interleukin-22+CD4+CD161+ T cells in rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2019.02.006
- VernacularTitle:类风湿关节炎患者外周血白细胞介素-22+CD4+CD161+T细胞频数变化
- Author:
Pin WU
1
;
Lijun YAN
;
Jing LIU
;
Dongmei GAO
;
Qian WANG
;
Jie HU
;
Nanfang CHEN
;
Wenjing XU
;
Zhequn SHI
;
Hejiang YANG
;
Shengquan TONG
;
Chunfen LI
Author Information
1. 河北省唐山市工人医院风湿科 063000
- Keywords:
Arthritis,rheumatoid;
CD4-positive T-lymphocyetes;
CD161-positive T-lymphocyets;
Interferon-gamma;
Interleukin-22
- From:
Chinese Journal of Rheumatology
2019;23(2):102-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the frequency of interleukin (IL)-22+CD161+CD4+ T cells in the peripheral blood mononuclear cells (PBMCs) in rheumatoid arthritis (RA) patients compared with healthy control subjects and investigate the relationship of IL-22+CD4+CD161+ T lymphocyte frequency changes with RA disease activity.In addition to explore the pathogenesis of RA,and to look for new treatment targets for RA.Methods Twenty-one RA cases were included in the Department of Rheumatology of Tangshan Gongren Hospital from 2017 to 2018.Fourteen patients were female and 7 were male with the age ranged from 36 to 74 years old.The average age of this group of patients was (55±10) years,the average disease course was (60±50) months.All patients fulfilled the classification criteria of American College of Rheumatology [American College of Rheumatology (ACR)].Twenty-one subjects were enrolled as the control group,all of them came to Tangshan Gongren Hospital for regular health check-up.Fifteen subjects in the control group were female and 6 were male.Their age ranged between 40-78 years old with the average age of (55±9) years.IL-22+CD4+CD161+ T cells in PBMCs were detected by flow cytometry.The frequency variation of different CD4+CD161 + T was compared between case and control groups.The correlation was studied between the frequency and RA disease activity score (DAS28),tender joints number,swollen joints number,red blood cell sedimentation rate,high sensitive C reactive protein and white blood cell counts,red blood cell counts,platelet counts,IgG,IgA,IgM,complement C3 level,complement C4 level.T-test or Mann-Whitney U test were used for single-factor analysis,Pearson's test was used for correlation analysis.Results The percentage of RA group secreted CD4+ T cells (0.33± 0.20)％ of INF-γand IL-22,CD4+ T cells (0.51±0.29)％ of IL-22,and CD4+CD161+ T cells of IL-22 simultaneously.The number (0.55 ±0.28)％ was.significantly higher than that of the healtby control group [(0.22±0.14)％,(0.25±0.18)％,(0.36±0.24)％],and the differences were statistically significant [P=0.002,P=-0.0.45,P=0.026].Conclusion The percentage of IL-22+CD4+CD161+ T lymphocytes in the peripheral blood monocytes in RA patients is significantly higher than that in the healthy controls.The results of this study suggest that IL-22+CD4+CD161+ T lymphocytes in RA patients maybe related to RA disease activity and joint lesions.
