Experimental study of IL-8 monoclonal antibody combined with ultrasound targeted microbubble destruction on myocardial ischemia/reperfusion injury in rabbits
10.3760/cma.j.issn.1004-4477.2019.01.016
- VernacularTitle:IL-8单克隆抗体联合超声靶向微泡破坏技术对兔心肌缺血/再灌注损伤的实验研究
- Author:
Yue SUN
1
;
Lirong XU
;
Guimin ZHU
;
Hanning YANG
;
Lihong YANG
;
Yongping LU
Author Information
1. 昆明医科大学第四附属医院 云南省第二人民医院超声科650021
- Keywords:
Ultrasound targeted microbubble destruction;
Myocardial contrast echocardiography;
Myocardial ischemia-reperfusion injury;
Interluekin-8;
Microbubbles
- From:
Chinese Journal of Ultrasonography
2019;28(1):77-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application value of IL-8 monoclonal antibody microbubble combined with ultrsound targeted microbubble destruction ( UTMD) on alleviating myocardial ischemia reperfusion/injury ( MIRI) in rabbits .Methods Forty-two rabbits were randomly divided into closed chest group ( n =7) ,open chest control group ( n = 7) and ischemia-reperfusion ( I/R) group ( n = 28) .I/R group were randomly divided into 30 min reperfusion group( n =7) ,60 min reperfusion group( n =7) ,120 min reperfusion group ( n = 7 ) and 180 min reperfusion group ( n = 7 ) .All rabbits were examined by electrocardiogram , echocardiography and HE staining after MIRI . Targeted myocardial contrast echocardiography ( MCE) was performed and ELISA was used to detect IL-8 content in rabbit myocardium before and after UTMD . Results Electrocardiogram and wall motion returned to normal at 60 min after reperfusion .Targeted MCE showed that with the prolongation of reperfusion after I/R ,the video intensity of myocardium in reperfusion area increased gradually , reaching its peak at 120 min and 180 min after reperfusion .After UTMD ,the video intensity decreased ,and the change rate of video intensity in 30 min reperfusion group was higher than those in other reperfusion groups(all P<0 .05) .The content of IL-8 and its neutralization rate in the ELISA results were consistent with the video intensity and rate of change of targeted MCE .HE staining and scanning electron microscopy showed that myocardial injury was found in I/R group .With the prolongation of reperfusion time ,the degree of myocardial injury was gradually aggravated ,and the injury was alleviated after irradiation .Conclusions IL-8 monoclonal antibody combined with UTMD has the advantages of non-invasive and highly effective in alleviating MIRI .It provides a new way to treat MIRI .
