Effect of long-chain non-coding RNA AL136359.1 on the proliferation and migration of cholangiocarcinoma cells by up-regulating the expression of AKR1B10
10.3760/cma.j.issn.1008-1372.2019.04.012
- VernacularTitle:长链非编码RNA AL136359.1通过上调AKR1B10的表达对胆管癌细胞增殖和迁移的影响
- Author:
Min CAI
1
;
Liu XU
;
Lan SHEN
;
Jie ZHANG
Author Information
1. 嘉兴市第一医院普外科 314001
- Keywords:
Bile duct neoplasms;
RNA,long noncoding;
Cell proliferation;
Cell migration;
In vitro
- From:
Journal of Chinese Physician
2019;21(4):532-535
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of AL136359.1 in cholangiocarcinoma tissues and cells and analyze its effect on proliferation and migration of cholangiocarcinoma cells.Methods The expression of AL136359.1 in 8 cholangiocarcinoma tissues and 4 cholangiocarcinoma cell lines (QBC939,RBE,HCCC-9810 and TFK-1) was detected by real-time quantitative PCR (qRT-PCR).Transfection of plasmid carrying AL136359.1 in the cholangiocarcinoma cell line with the lowest expression level of AL136359.1 were used to increase the expression of AL136359.1,and the transfection efficiency was verified by qRT-PCR.qRT-PCR and Western blot were used to detect the expression of AKR1 B10 at mRNA and protein levels.The cell counting kit (CCK-8) and transwell migration assay were used to detect the effect of high expression of AL136359.1 on the proliferation and migration ability of cholangiocarcinoma cells.Results The expression of AL136359.1 in cholangiocarcinoma tissues (0.41± 0.05) was significantly lower than that in adjacent tissues (2.94 ± 0.44,P ＜ 0.01).The expression of AL136359.1 in cholangiocarcinoma cells was significantly lower than that in human normal cholangioepithelial cells (P ＜0.01),and the QBC939 cells had the lowest expression (P ＜ 0.01).High expression of AL136359.1 caused an increase in the expression of AKR1 B10 at mRNA and protein levels (P ＜ 0.01).High expression of AL136359.1 could significantly inhibit the proliferation and migration of cholangiocarcinoma cells (P ＜0.01).Conclusions The expression of AL136359.1 were decreased in cholangiocarcinoma tissues and cell line.The high expression of AL136359.1 could up-regulate the mRNA expression of AKR1B10 gene,which was involved in inhibiting the proliferation and migration of QBC939 cells.
