Expression and clinical significance of autophagy-related proteins LC3, P62 and Beclin-1 in hypertrophic scar formation
10.3760/cma.j.issn.1008-1372.2019.04.003
- VernacularTitle:增生性瘢痕形成过程中自噬相关蛋白LC3、P62及Beclin-1的表达及临床意义
- Author:
Xiaoxia CHEN
1
,
2
;
Siwei QU
;
Xiao HAN
;
Zizi CHEN
;
Jia CHEN
;
Ping LI
;
Chaoqi YIN
;
Sainan ZENG
;
Aijun WANG
;
Bin HE
;
Honghui SU
;
Jianda ZHOU
Author Information
1. 湖南宁乡市人民医院整形美容外科 410600
2. 中南大学湘雅三医院整形美容外科
- Keywords:
Cicatrix,hypertrophic;
Microtubule-associated proteins;
Beclin-1;
RNA-binding proteins;
Autophagy;
Rabbits
- From:
Journal of Chinese Physician
2019;21(4):489-494
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the differences in autophagic expression levels between hypertrophic scar (HS) tissue and normal skin tissue,and further investigate the relationship between hypertrophic scar formation and autophagy protein expression through the rabbit ear hypertrophic scar model.Methods 30 patients with hypertrophic scar were collected.One hypertrophic scar tissue and one normal skin tissue were harvested.The relative expressions of LC3,P62 and Beclin-1 in each tissue specimen were detected by immunohistochemistry and Western blot.Western blot was used to detect the autophagic-associated protein LC3 (MAPLC3),P62 and Beclin-1 in the hypertrophic scar tissue of rabbit ear and the corresponding normal tissue of rabbit ears at 4 weeks,8 weeks,12 weeks,and 24 weeks,and further explore their clinical significance.Results In vivo,the expression of hypertrophic scar tissue protein LC3 and Beclin-1 was significantly stronger than that in normal skin tissue (P ＜ 0.05).The expression of P62 was significantly weaker than that in normal skin tissue (P ＜ 0.05).In animal experiments,during the process of HS formation,the protein expression of LC3 gradually increased,while the protein expression of P62 gradually decreased;the protein expression of Beclin-1 was higher than that of normal rabbit ears tissue,with statistically significant differences (P ＜ 0.05).Conclusions The expression of LC3 and Beclin-1 in human hypertrophic scar tissues is higher than that in normal tissues.While the expression of P62 is lower than that in normal tissues.That is,the expression of autophagy in human hypertrophic scar tissue showed an upward trend in a certain period of time,and was significantly higher than that in normal tissue.
