Effect of p53 mutation mediated by APOBEC3B on NSCLC metastasis

VernacularTitle:APOBEC3B介导的p53突变在NSCLC转移中的作用
Author: Jiahui YE ¹ ; Yongsheng CHEN ; Kai LUO ; Zhimin HE ; Yixue GU
Author Information

1. 广州医科大学附属肿瘤医院肿瘤研究所 510095
Keywords: Minor histocompatibility antigens; Cytidine deaminase; Genes,p53; Carcinoma,non-small-cell lung; Neoplasm metastasis
From: Journal of Chinese Physician 2019;21(3):373-378
CountryChina
Language:Chinese
Abstract: Objective APOBEC3B (A3B) is an important member of the apolipoprotein B mRNA editing enzyme catalytic polypeptide (APOBEC) family.This study aimed to investigate its important role in the metastasis of small cell lung cancer (NSCLC).Methods The statistical relationship between A3 B and clinicopathological data was analyzed in 249 cases of NSCLC.Sanger sequencing was used to detect mutations in exon 5,6,7 and 8 of P53 in 74 cases of lung cancer.A3B overexpression cell line was constructed in human lung adenocarcinoma cells HCC827 to observe the change of cell migration and metastasis capacity.Results A3B was highly expressed in NSCLC tissues compared with normal lung tissues.The expression of A3B was closely related to the lymph node metastasis of NSCLC and the mutation rate of p53 was positively correlated with the expression of A3B.In vitro experiment,it showed enhanced migration and increased metastatic potential in cells after overexpression of A3B.Conclusions A3B-mediated mutations in P53 may play a key role in the metastasis of NSCLC.