Effects and mechanism of Momordica charantia MAP30 on migration of bladder cancer
10.3760/cma.j.issn.1008-1372.2019.02.007
- VernacularTitle:苦瓜Ⅰ型核糖体失活蛋白对膀胱癌迁移能力的影响及机制研究
- Author:
Kun PANG
1
;
Bo CHEN
;
Lin HAO
;
Zhenduo SHI
;
Rongsheng ZHOU
;
Guanghui ZANG
;
Conghui HAN
Author Information
1. 东南大学附属徐州市中心医院泌尿外科 221000
- Keywords:
Ribosome inactivating proteins;
type 1;
Urinary bladder neoplasms;
Cell migration assays;
Epithelial-mesenchymal transition;
In vitro
- From:
Journal of Chinese Physician
2019;21(2):184-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and mechanism of Momordica anti-HIV protein of 30 ku (MAP30) on the migration of bladder cancer.Methods The IC50 of human bladder cancer 5637 and T24 cells was calculated by methyl thiazolyl tetrazolium (MTT) method.The migration ability of these two cells was evaluated by scratch migration test and Transwell cell migration test.The expression of migrating proteins such as matrix metalloproteinases (MMPs) and adhesion molecule N-cadherin were compared by Western blot.Results Scratch migration test:there were significant differences in migration rates of 5637 cells at 8 h and 22 h (P ＜ 0.05).There were significant differences in migration rates of T24 cells at 22 h (P ＜ 0.05),but no significant differences in migration rates at 8 h (P ＞ 0.05).The expression of Vimentin,Fibronectin,MMP-2,MMP-9 and N-Cadherin in 5637 cells and T24 cells of human bladder cancer decreased significantly after adding MAP30.The E-Cadherin expression in human bladder cancer 5637 cells were decreased,but no target band was detected in human bladder cancer T24 cells.Conclusions The ribosome-inactivating protein MAP30 can effectively inhibit the migration of human bladder cancer 5637 and T24 cells by inhibiting the EMT pathway and inhibiting the expression of MMPs.
