Genistein Inhibits the Proliferation of Human Lung Cancer PC14 Cells through the ERK and JNK Pathways
- VernacularTitle:染料木黄酮通过ERK及JNK通路抑制人非小细胞肺癌PC14细胞增殖
- Author:
Xuemei JIANG
1
;
Yue QIAN
;
Hongliang DAI
Author Information
1. 锦州医科大学附属第一医院消化科
- Keywords:
genistein;
PC14 cells;
proliferation;
ERK;
JNK
- From:
Journal of China Medical University
2019;48(3):274-276,280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of genistein on the proliferation of human lung cancer PC14 cells and the underlying mechanisms. Methods Cell proliferation was examined using the MTT and colony formation assays. Western blotting was used to analyze protein expression levels. Results Genistein significantly inhibited the proliferation of PC14 cells in a concentration and time dependent manner. PD98059, SB203580, and SP100625, three specific inhibitors of the MAPK pathway, significantly inhibited the proliferation of PC14 cells. Moreover, genistein inhibited the phosphorylation of ERK and JNK in a dose dependent manner. Conclusion Genistein can inhibit the proliferation of PC14 cells, which may be related to its inhibitory effect on ERK and JNK activation.
