miR-362-3p Expression in Laryngeal Cancer Tissues and Its Effect on Migration of Hep-2 Cells
- VernacularTitle:miR-362-3p在喉癌组织中的表达及其对喉癌Hep-2细胞迁移的影响
- Author:
Yue ZHAO
1
;
Yuanyuan SUN
;
Xue TONG
;
Pengjie YUE
;
Weineng FU
Author Information
1. 中国医科大学基础医学院遗传学教研室
- Keywords:
miR-362-3p expression;
laryngeal cancer;
migration
- From:
Journal of China Medical University
2019;48(3):236-239
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore miR-362-3 p expression in laryngeal cancer tissues and its effect on migration of Hep2 cells. Methods miR-362-3 p expression in 50 pairs of tumor and adjacent normal tissues was detected by real-time PCR after sample collection. The relationships between miR-362-3 p expression and clinical pathological characteristics in patients with laryngeal cancer were analyzed.mi R-362-3 p mimic, inhibitor, and control microRNA were transfected into Hep-2 cells. Transfection efficiency was determined by real-time PCR. Wound-healing and transwell assays were used to evaluate Hep-2 migration. Results miR-362-3 p expression was significantly higher in cancer tissues than in adjacent normal tissues (P ＜ 0.05). miR-362-3 p expression was statistically significantly related to node metastasis and clinical stage. Transfection with miR-362-3 p mimic and inhibitor significantly increased and decreased Hep-2 cell migration, respectively (both P ＜ 0.05). Conclusion miR-362-3 p is up-regulated in laryngeal cancer tissues and promotes laryngeal cancer cell migration, suggesting that it acts as a potential oncogene in laryngeal cancer.
