miR-24 Inhibits Lung Cancer Cell Proliferation and Migration
- VernacularTitle:miR-24对肺癌生物学功能的抑制作用
- Author:
Liming WANG
1
;
Daye YANG
;
Jian QIU
;
Dewei ZHANG
Author Information
1. 中国医科大学附属第四医院第三普通外科
- Keywords:
miR-24;
lung cancer;
A549;
proliferation;
migration
- From:
Journal of China Medical University
2019;48(1):71-74
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate how microRNA 24 (miR-24) regulates the development of lung cancer. Methods The levels of miR-24 in 30 cases of lung cancer were detected using real-time PCR, and the relationship between miR-24 levels and overall survival was analyzed. After overexpression or silencing of miR-24 in A549 lung cancer cells, the effect on cell proliferation was observed by the MTT assay. Transwell assays were carried out to observe the effect of miR-24 on cell migration. The effects of miR-24 on the expression of cyclin-dependent kinase (CDK) 4/6 and matrix metalloproteinase (MMP) 2 in A549 cells were examined by Western blotting. Results Survival analysis showed that patients with low miR-24 expression had a shorter survival time. The MTT-based viability assay revealed that overexpression of miR-24 inhibited A549 cell proliferation. Cell proliferation was promoted when miR-24 was inhibited. In the Transwell assay, overexpression of miR-24 significantly inhibited the A549 cell migration, and cell migration increased when miR-24 was inhibited.Western blotting analysis revealed that overexpression of miR-24 could inhibit CDK4/6 and MMP2 production in A549 cells. Inhibition of miR-24 was associated with increased production of CDK4/6 and MMP2 in A549 cells. Conclusion miR-24 can inhibit the proliferation and migration of A549 lung cancer cells.
