Effects of Artesunate on Invasion and Metastasis of Human Colon Cancer Cells and the TGF-β1/Smad4 Signaling Pathway
- VernacularTitle:青蒿琥酯对人结肠癌细胞侵袭转移及TGF-β1/Smad4信号通路表达的影响
- Author:
Song LIU
1
;
Fang QI
;
Yu ZHAO
;
Keyan CHEN
;
Shengrui ZHANG
Author Information
1. 沈阳医学院附属中心医院普外科
- Keywords:
artesunate;
colon cancer;
invasion and metastasis;
TGF-β1/Smad4
- From:
Journal of China Medical University
2019;48(1):34-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism via which artesunate regulates the invasion and metastasis of colon cancer cells and the expression of members of the TGF-β1/Smad4 signaling pathway. Methods The cell counting kit 8 (CCK8), nude mouse xenograft model, Transwell invasion assay, and flow cytometry were used to investigate the effect of artesunate on the invasion and metastasis of colon cancer cells. Western blotting and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were used to detect the expression of TGF-β1 and Smad4 proteins and mRNA, respectively. Results Artesunate inhibited the growth of transplanted tumor, cell proliferation, and invasion and promoted apoptosis. It inhibited TGF-β1 expression and promoted Smad4 expression. TGF-β1 inhibitors reversed the inhibitory effect of artesunate. Conclusion Artesunate can inhibit the growth of xenograft tumor in nude mice and its mode of action may be related to the TGF-β1/Smad4 signaling pathway.
