Effect of miR-200c Regulation of Peptidyl-Prolyl Cis/Trans Isomerase on the Biological Behavior of Hep-2 Cells
- VernacularTitle:miR-200c调控肽基脯氨酰顺反异构酶对喉癌Hep-2细胞生物学行为的影响
- Author:
Xing WEN
1
;
Zhangfu LI
;
Hui WANG
;
Shaohua SUN
;
Xing GUO
;
Fucai LI
Author Information
1. 中国医科大学基础医学院医学遗传学教研室
- Keywords:
laryngeal squamous cell carcinoma;
miR-200c;
peptidyl-prolyl cis/trans isomerase;
migration;
centrosome
- From:
Journal of China Medical University
2019;48(1):17-22,28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of miR-200 c on the biological behavior of laryngeal carcinoma Hep-2 cells and determine whether miR-200 c exerts its biological function through peptidyl-prolyl cis/trans isomerase (PIN1) in laryngeal carcinoma. Methods A qRT-PCR assay for the expression of miR-200 c was performed in laryngeal carcinoma tissues. Hep-2 cells were transfected with miR-200 c related small RNAs. Transwell assay detected the migration ability of the cells. Immunofluorescence assay was used to detect the abnormal amplification of the centrosome. A dual luciferase reporter gene system was used to detect the binding ability between miR-200 c and PIN1. Western blotting detected the protein expression level of PIN1. Results The expression of miR-200 c in laryngeal carcinoma was significantly increased. miR-200 c inhibited the migration of Hep-2 cells and could weaken the abnormal amplification of centrosome.PIN1 was confirmed as one of the target genes of miR-200 c. miR-200 c inhibited the expression of PIN1 at the translation level and could inhibit Hep-2 cell migration and abnormal centrosome amplification by regulating PIN1. Conclusion miR-200 c can inhibit the migration ability of laryngeal carcinoma cells and abnormal centrosome amplification by regulating PIN1.
