The effect of moderate running wheel exercise on the learning and memory ability and hippocampal neurogenesis in young mice
10.3760/cma.j.issn.1674-6554.2019.04.007
- VernacularTitle:适度跑轮运动对幼年小鼠学习记忆能力及海马神经发生的影响
- Author:
Yang SHI
1
;
Bingzhang LI
;
Cuili CAO
Author Information
1. 河北医科大学基础医学院神经生物学研究室
- Keywords:
Moderate running wheel exercise;
Morris water maze test;
Learning and memory;
Hippocampal;
Neurogenesis;
Young mice
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2019;28(4):322-325
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of moderate running wheel exercise on the learning and memory ability and hippocampal neurogenesis in young mice.Methods Twenty male 1 month old Kunming mice were randomly divided into control group and exercise group.After 8 weeks of running wheel exercise in the exercise group,the Morris water maze test was used to detect the spatial learning and memory ability of the two groups of mice.Immunohistochemical technique was used to detect the expression of Sox2,Ki67 and DCX in the dentate gyrus of mice in two groups,and those specific protein can reflected the hippocampal neurogenesis.Results In the place navigation test of Morris water maze,the latency of the exercise group ((29.00± 1.32) s) was lower than that of the control group ((36.30±0.69) s),and the difference was statistically significant (t=5.154,P＜0.05).In the spatial probe test,the number of times of crossing platforms in the exercise group ((3.73±1.51) times) was more than that of the control group ((1.89±t 1.63) times),and the difference was significant (t=3.583,P＜0.05).Immunohistochemical results showed that the number of Sox2,Ki67 and DCX immunoreactive cells in the dentate gyrus region of the exercise group were ((284.40± 31.50),(54.50± 10.75),(77.80=t± 11.60) respectively) more than those in the control group ((241.40± 10.57),(37.00± 7.81),(48.20±t 11.86) respectively),and the difference was statistically significant (t =4.129,5.789,7.971,all P＜0.01).Conclusion Moderate running wheel exercise can significantly improve the learning and memory ability of young mice,which may be related to the promotion of neurogenesis in the dentate gyrus of the hippocampus.
