Effect of STC-1 gene expression was inhibited on cell apoptosis, IL-1β and TNF-α expression and JAK2/STAT3 signal in esophageal carcinoma
10.3969/j.issn.1000-484X.2019.02.012
- VernacularTitle:抑制食管癌STC-1基因表达对食管癌细胞凋亡、IL-1β和TNF-α表达及JAK2/STAT3信号的影响研究
- Author:
Xiu-Mei BU
1
;
Wen-Gang WANG
;
Hui LI
;
Jin ZHENG
Author Information
1. 辽宁中医药大学
- Keywords:
Esophageal cancer;
STC-1 gene;
Apoptosis;
Immunization;
JAK2/STAT3 signaling pathway
- From:
Chinese Journal of Immunology
2019;35(2):186-191
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of STC-1 gene expression was inhibited on the apoptosis, IL-1β and TNF-α expression and JAK2/STAT3 signal in esophageal cancer cells. Methods: Compared with normal human esophageal squamous epithelial cells Het-1 A, STC-1 expression was detected in human esophageal squamous cell carcinoma KYSE170, Eca109, TE1 and TE10 cells by RTPCR and Western blot; the siRNA sequence that the synthesized STC-1 and the si NRA sequence without interference were transfected into Eca109 cells, which were labeled as STC-1-siRNA group and NC group, and the blank control group was set, cells were transfected for 48 h, the expression of STC-1 were detected by RT-PCR and Western blot. Eca109 cell viability and apoptosis rate were detected by CCK8 and flow cytometry. IL-1β and TNF-α expression were detected by RT-PCR; the expression of Ki67, p53, p-JAK2 and p-STAT3 protein were detected by Western blot. Results: Compared with Het-1 A cells, expression of STC-1 mRNA and protein in KYSE170, Eca109, TE1 and TE10 cells were increased significantly ( P＜0. 05); compared with the control group, STC-1 expression was decreased significantly in STC-1-siRNA group, cell viability was decreased significantly in STC-1-siRNA group, the apoptosis rate was increased significantly in STC-1-siRNA group; IL-1β, TNF-α, Ki67, p-JAK2 and p-STAT3 expression were decreased significantly in STC-1-siRNA group, p53 expression was increased significantly in STC-1-siRNA group ( P＜0. 05). Conclusion: STC-1 was highly expressed in esophageal cancer cells; inhibiting of STC-1 expression could significantly reduce the activity of cancer cells, and increase apoptosis rate, this effect may be related to the inhibition of JAK2/STAT3 signaling pathways and inflammatory factors as IL-1β and TNF-α.
