Effects of curcumin on spinal astrocyte damage induced by H2O2 through Notch signaling pathway
10.3969/j.issn.1000-484X.2019.02.008
- VernacularTitle:姜黄素通过Notch信号通路对H2O2诱导的脊髓星形胶质细胞损伤的影响研究
- Author:
Feng GAO
1
;
Lin ZHAO
;
Yan-Ling YANG
Author Information
1. 延安大学医学院
- Keywords:
Curcumin;
Spinal cord injury;
Apoptosis;
Notch signaling pathway
- From:
Chinese Journal of Immunology
2019;35(2):165-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of curcumin on Asrocytes and Notch signaling pathway in spinal cord injury. Methods Spinal cord Asrocytes was isolated and cultured, and cells were treated with 0. 1, 0. 2, 0. 4, 0. 6, 0. 8, 1 mmol/L hydrogen peroxide ( H2O2), the cell viability was measured by CCK8, AS damage model was established.Methods: The experimental grouping was control group, H2O2 group and H2O2+curcumin group, cell apoptosis and ROS content were detected by flow cytometry; the content of IL-6 and TNF-α were detected by ELISA kit; the expression of Cleaved Caspase3, Bax, Notch1 and Hes1 protein were detected by Western blot. Results: The activity of AS was inhibited by different concentrations of H2O2, and the inhibition rate of cell increased with the increase of H2O2 concentration. IC50 for 0. 4 mmol/L's H2O2 was selected to establish a damage model; compared with the normal control group, the apoptosis rate, the content of ROS, IL-6, TNF-α and expression of Cleaved Caspase3, Bax, Notch1 and Hes1 protein expression were increased significantly in H2O2 group, compared with H2O2 group, the apoptosis rate, the content of ROS, IL-6, TNF-α and expression of Cleaved Caspase3, Bax, Notch1 and Hes1 protein expression were lower significantly in H2O2+curcumin group ( P＜0. 05). Conclusion: Curcumin can reduce the apoptosis of Asrocytes, the content of ROS and the contents of inflammatory factors IL-6 and TNF-α in spinal cord by down regulating the Notch signaling pathway, thereby protecting spinal cord injury.
