Correlation of HBV genotyping and drug resistance mutation in Hohhot,Inner Mongolia
10.3760/cma.j.issn.1008-6706.2019.08.015
- VernacularTitle:内蒙古自治区呼和浩特地区乙型肝炎患者HBV基因分型、耐药突变的相关性分析
- Author:
Xiaohua ZHENG
1
;
Pengwei ZHAO
;
Haiqin JIA
Author Information
1. 内蒙古自治区人民医院检验科
- Keywords:
Hepatitis B,chronic;
Hepatitis B virus;
Genes,viral;
Deoxyribonucleic acid;
Hepatitis B e antigens;
Hepatitis B surface antigens;
Hepatitis B core antigens;
Genotype;
Drug resistance mutation
- From:
Chinese Journal of Primary Medicine and Pharmacy
2019;26(8):956-959
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between HBV -DNA load and serum markers in chronic hepatitis B( CHB) patients in Hohhot,Inner Mongolia,and to explore the mutation of HBV genotype and nucleoside analogue.Methods From January 2015 to December 2017,one hundred and ninety-three CHB patients hospitalized in the People＇s Hospital of Inner Mongolia were selected randomly.The clinical diagnostic criteria for all admitted patients were based on the " Guidelines for the Prevention and Treatment of Chronic Hepatitis B" jointly formulated by the Infectious Diseases Society of 2010. The HBV -DNA load of HBV was detected by real -time quantitative PCR,and the correlation between HBV -DNA load and serum markers was analyzed. Seventy -nine patients were selected from 193 hospitalized patients,PCR-reverse dot blot hybridization was used to analyze HBV genotyping and the drug resistance mutations of different genotypes.Results The differences of HBeAb level and HBV-DNA load between HBeAg positive patients and negative patients were statistically significant(all P<0.001). Of 79 serum specimens of HBV infected people,9 cases(11.4% ) were B genotypes,and 70 cases of C genotype (88.6% ).Of them,25 cases had different loci variation,the rate of variation was 31.6% (25/79),with the unit point rtS213T mutation dominated,accounting for about 24.0% (6/25).Conclusion In Hohhot Inner Mongolia patients with CHB,HBV-DNA load with HBeAg and HBe Ab level are correlated;genotype in patients including B type and C type,which is mainly genotype C;patients with CHB mainly had drug resistance to lamivudine and adefovir dipivoxil, mutations including rtS213T,and hybrid mutation.
