Expression of SPARC in smooth muscle tissue of ob/ob mice and its clinical significance
10.3760/cma.j.issn.1008-6706.2019.04.001
- VernacularTitle:ob/ob小鼠平滑肌组织中富含半胱氨酸的酸性分泌蛋白的表达及临床意义
- Author:
Xiaoyao YANG
1
;
Yiwei WANG
Author Information
1. 黑龙江省医院科教部
- Keywords:
Secreted protein acidic and rich in cysteine;
Mice;
Artery smooth muscle tissue;
Diabetes;
Mesentery;
Expression;
Atherosclerosis
- From:
Chinese Journal of Primary Medicine and Pharmacy
2019;26(4):385-387
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the secreted protein acidic and rich in cysteine (SPARC) expression in the smooth muscle tissue of ob/ob mice.Methods Study time:February 2017 to June 2017.Six ob/ob mice and 6 littermates aged 10 weeks were selected for the trial.The mesenteric artery smooth muscle tissues were collected.qRT -PCR was used to detect SPARC mRNA expression in smooth muscle tissue.The protein expression of SPARC was assayed by Western blot.Results The SPARC mRNA [(1.73 ± 0.65)] and protein [(1.73 ± 0.65)] in smooth muscle tissue of ob/ob mice presented higher expression compared with those in the littermates [(1.00 ± 0.31),(1.00 ± 0.33),t =8.437,5.533,all P ＜ 0.05].Conclusion The higher expression of SPARC in smooth muscles tissue of ob/ob mice may be involved in diabetes combined with atherosclerosis.
