Molecular characterization of the Brucella ovis isolated in Nanjing
10.3760/cma.j.issn.2095-4255.2019.04.005
- VernacularTitle:南京市羊种布鲁菌分子特征分析
- Author:
Ying ZHANG
1
;
Zhifeng ZHANG
;
Jie HONG
;
Weizhong ZHOU
;
Changjun BAO
;
Minghao ZHOU
;
Zhongming TAN
Author Information
1. 南京大学医学院附属鼓楼医院产前诊断中心 210008
- Keywords:
Brucella;
Multilocus sequence typing;
Multiple locus variable-number tandem repeat analysis
- From:
Chinese Journal of Endemiology
2019;38(4):278-281
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the molecular characteristics of Brucella strains isolated from Nanjing,understand strains genotying and clustering,and to provide a basis for prevention and treatment of brucellosis.Methods Multilocus sequence typing (MLST) and multiple locus variable-number tandem repeat analysis (MLVA) were used to analyze and characterize Brucella ovis strains isolated from 7 cases of sporadic cases in Nanjing Drum Tower Hospital,the Affiliated Hospital of Nanjing University Medical School,from 2011-2016,and cluster analysis was did with reference strain data from Jiangsu Province.Results The results showed that 7 strains were defined as sequence type (ST) 8 by MLST.They were typed into 7 subtypes and clustered in the "Middle Mediterranean Cluster" by MLVA.Strain NJ-2011-1 and two strains isolated from other cities in Jiangsu had the same MLVA genotype.Conclusions The results reveal ST8 is the predominant genotype in Nanjing.They have clustered in the "Middle Mediterranean Cluster" by MLVA.The 7 strains are sporadic.The transmission routes and risk factors are more complicated in the city.Various departments should strengthen the cooperation to control the source.
