Molecular typing of Brucella abortus strains in Xinjiang

VernacularTitle:新疆地区牛种布鲁菌流行株的分子分型鉴定
Author: Feng YE ¹ ; Xiaojing MA ; Liya LIU ; Caiyun XIE ; Wenxi GU ; Qi ZHONG ; Junjie MA ; Xinping YI
Author Information

1. 新疆畜牧科学院兽医研究所
Keywords: Brucella; Biological assay; Bacterial typing techniques
From: Chinese Journal of Endemiology 2019;38(4):265-268
CountryChina
Language:Chinese
Abstract: Objective To identify molecular typing of Brucella abortus isolates in Xinjiang,and determine the identification ability of multiple locus variable-number tandem repeat analysis (MLVA).Methods The optimized Brucella AMOS-PCR was used for identification of Brucella (n =7) genus and species in Xinjiang from 2010-2015,and MLVA-16 was used to further identify the isolates.Results were compared with the data of the Brucella standard strain provided by the http://mlva.u-psud.fr database.Cluster analysis was carried out with Bionumerics 6.6.Results The results of AMOS-PCR and MLVA-16 were identical,all were Brucella abortus.Further classification results of the MLVA-16 showed that the strain in Xinjiang was type 3 of Brucella abortus,which was basically the same as that of the domestic Brucella.Conclusions The molecular typing of isolates separated in Xinjiang is type 3 of Brucella abortus.MLVA can identify Brucella at the level of species,and highly sensitive to Brucella biotype and isolates differences,which provides a basis for the traceability and evolution of brucellosis epidemic strains.