Pigment epithelium——derived factor is involved in the vascular endothelial cells dysfunction induced by arsenic
10.3760/cma.j.issn.2095-4255.2019.02.005
- VernacularTitle:色素上皮衍生因子参与砷诱导的血管内皮细胞功能失调
- Author:
Wei ZHANG
1
;
Lijun ZHAO
;
Dean LI
;
Xiangnan GUO
;
Jingqiu WANG
Author Information
1. 哈尔滨医科大学中国疾病预防控制中心地方病控制中心碘缺乏病防治研究所 150081
- Keywords:
Arsenites;
Nitric oxide;
Vascular endothelial cell;
Pigment epithelium-derived factor
- From:
Chinese Journal of Endemiology
2019;38(2):107-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of pigment epithelium-derived factor (PEDF) in vascular endothelial cells dysfunction induced by sodium arsenite (NaAsO2).Methods Human umbilical vein endothelial cells (EA.Hy926 cells) were treated with different levels of NaAsO2 [0 (control),1,2,5,10,20,50 μmol/L] for 24 hours.The cell viability was determined using CCK8.Colorimetric assay was used to detect the activity of inducible nitric oxide synthase (iNOS) in culture supematants,PEDF content in the supematant of EA.Hy926 cells was detected by enzyme-linked immunosorbent assay (ELISA),and nitric oxide (NO) content in cells was detected by flow cytometry.Results Compared with the control group [(101.08 ± 3.22)％],the cell viability of 20 μ mol/L group [(80.69 ± 7.95)％] and 50 μmol/L group [(69.87 ± 10.54)％] decreased significantly,and the differences were statistically significant (P ＜ 0.05).The activity of iNOS increased significantly in 10,20,50 μmol/L groups [(829.1 ± 68.2),(772.3 ± 37.1),(874.6 ± 43.5) U/L],respectively,in comparison with that of the control group [(397.5 ± 43.5) U/L] in the cell culture supematant (P ＜ 0.01).Similarly,PEDF levels in the groups of 10,20,50 μmol/L [(12.06 ± 0.55),(11.97 ± 0.39) and (13.89 ± 0.26) mg/L respectively] were higher than that of the control group [(10.70 ± 0.35) mg/L,P ＜ 0.01],and the highest content of PEDF was found in 50 μmol/L group.The NO level in 50 μmol/L group (11 558.99 ± 397.43) was significantly lower than that of the control group (14 131.49± 262.61,P ＜ 0.01).Conclusion PEDF is involved in the vascular endothelial cells dysfunction induced by arsenic.
